β-Mercaptoethanol Enhances Blastocyst Formation Rate of Bovine in Vitro-Matured/in Vitro-Fertilized Embryos1
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چکیده
منابع مشابه
in vitro application of matrigel enhances human blastocyst formation and hatching
background: matrigel (extracellular matrix) can improve the growth of many cell types in vitro. objective: the aim of the present study was to determine the effect of matrigel on the development of 2-4 cells human embryos in culture. material and methods: surplus 2-4 cells human embryos, resulting from icsi, were divided into two groups (control and test). quality of embryos in both groups was ...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
In Vitro Cultivation of Early Rat Embryos1
In vitro cultivation of early mammalian embryos under controlled con ditions in various kinds of media have been reported by numerous investigators. According to Austin's detailed review in 1961, the majority of studies in this field have involved the rabbit, mouse, and a few other mammalian species. It has been shown that rabbit ova will cleave readily and develop into morulae in a defined med...
متن کاملStimulation of development of in vitro-matured and in vitro-fertilized bovine embryos by platelets.
In vitro-fertilized bovine embryos were incubated in Menezo's B2 medium (MB2) supplemented with 2 mg/mL of BSA. In Exp. 1, eight-cell stage embryos were allotted to one of the following groups: control medium (MB2), MB2 with 20 ng/mL of platelet-activating factor (PAF), 1 x 10(7) bovine blood platelets (Platelets), oviductal cells (BOEC), BOEC and 20 ng/mL of PAF (BOEC+PAF), or BOEC and 1 x 10(...
متن کاملIn vitro culture of bovine egg fertilized either in vivo or in vitro.
Three-quarters of in vivo and one-third of in vitro fertilized bovine eggs reached blastocyst stage when cultured on tubal cell monolayers (TCM), but no hatching occurred in B2 medium supplemented with estrous cow serum. When after 3 days of culture on TCM, morulae were transferred on endometrial cell monolayers (UCM), the same proportion of blastocysts was obtained and one-third of them hatche...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1996
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod55.5.1179